RT Book, Section
A1 Krishnamurthy, Savitri
A2 Kuerer, Henry M.
SR Print(0)
ID 6409975
T1 Chapter 24. Cytology in Breast Care Management
T2 Kuerer's Breast Surgical Oncology
YR 2010
FD 2010
PB The McGraw-Hill Companies
PP New York, NY
SN 978-0-07-160178-8
LK accesssurgery.mhmedical.com/content.aspx?aid=6409975
RD 2023/05/30
AB Cytopathology  is  a  specialized  branch  of  pathology  related  to  the  study  of  cells  derived  from  either  fine-needle  aspiration  biopsy  (FNAB)  or  exfoliation  including  effusions,  washings,  brushings,  or  body  fluids.  FNAB  is  performed  using  a  23-  or  25-gauge  hypodermic  needle  attached  to  a  10-mL  plastic  syringe.  FNAB  can  be  performed  blindly  for  palpable  masses  or  under  radiologic  guidance  including  ultrasound  (US),  computed  tomography  (CT),  or  magnetic  resonance  imaging  (MRI)  for  nonpalpable  lesions.  Specimens  obtained  from  any  of  these  sources  can  be  prepared  in  several  ways  for  conventional  cytopathologic  evaluation  and  ancillary  studies.  Smears  can  be  made  directly  or  from  the  cell  pellet  obtained  after  centrifugation  of  the  specimen  following  admixture  with  a  liquid  base  such  as  RPMI  or  Cytolyt  solution.  Monolayer  preparation  of  the  specimen  can  also  be  made  using  different  techniques  such  as  Thin  Prep  and  Sure  Path.  Smears  prepared  by  any  of  the  techniques  are  usually  fixed  in  alcohol  for  Papanicalaou  staining,  or  air-dried  for  Diff-Quik  staining.  In  addition,  aspirated  material  from  FNA  or  exfoliative  specimens  can  be  rinsed  in  RPMI  or  Cytolyt  solution  to  prepare  tissue  blocks  that  are  fixed  in  formalin,  processed  routinely  similar  to  surgical  tissues,  embedded  in  paraffin,  and  subsequently  cut  at  5  μm  and  finally  stained  by  the  hematoxylin  and  eosin  (H&E)  method.