TY - CHAP M1 - Book, Section TI - Chapter 24. Cytology in Breast Care Management A1 - Krishnamurthy, Savitri A2 - Kuerer, Henry M. PY - 2010 T2 - Kuerer's Breast Surgical Oncology AB - Cytopathology is a specialized branch of pathology related to the study of cells derived from either fine-needle aspiration biopsy (FNAB) or exfoliation including effusions, washings, brushings, or body fluids. FNAB is performed using a 23- or 25-gauge hypodermic needle attached to a 10-mL plastic syringe. FNAB can be performed blindly for palpable masses or under radiologic guidance including ultrasound (US), computed tomography (CT), or magnetic resonance imaging (MRI) for nonpalpable lesions. Specimens obtained from any of these sources can be prepared in several ways for conventional cytopathologic evaluation and ancillary studies. Smears can be made directly or from the cell pellet obtained after centrifugation of the specimen following admixture with a liquid base such as RPMI or Cytolyt solution. Monolayer preparation of the specimen can also be made using different techniques such as Thin Prep and Sure Path. Smears prepared by any of the techniques are usually fixed in alcohol for Papanicalaou staining, or air-dried for Diff-Quik staining. In addition, aspirated material from FNA or exfoliative specimens can be rinsed in RPMI or Cytolyt solution to prepare tissue blocks that are fixed in formalin, processed routinely similar to surgical tissues, embedded in paraffin, and subsequently cut at 5 μm and finally stained by the hematoxylin and eosin (H&E) method. SN - PB - The McGraw-Hill Companies CY - New York, NY Y2 - 2024/03/29 UR - accesssurgery.mhmedical.com/content.aspx?aid=6409975 ER -