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The liver is prepared for implantation (usually in a back table of the operating room where the transplant takes place) by:
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Removing unnecessary tissues
Preparing the vessels and biliary ducts for the future anastomoses
Making sure there are no defects in the vessels or biliary ducts
Identifying any potential lesions and/or injuries
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The sterile bag containing the liver and cold preservation fluid is placed within a basin with ice Figure 1.
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Stay sutures are placed on the proximal and distal ends of the inferior vena cava (IVC) Figures 2, 3, and 4. The triangular, coronary, and falciform ligaments are transected, allowing for the removal of the diaphragm.
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Venous branches (including phrenic, right adrenal, and lumbar veins) are tied and/or oversewn. A bulb syringe may be used to infuse preservation fluid (or alternatively air) through one end of the IVC (while clamping the other end) to detect any leaks. The entire length of the procured IVC is preserved, and any trimming is performed at the time of implantation.
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A cannula (preferably with a blunt edge) is placed in the portal vein and secured with a tie Figure 5. Tissues surrounding the portal vein are removed (at least around its free end) to allow for its subsequent anastomosis to its recipient counterpart. Branches are tied and/or oversewn.
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